Open Submitted: 12 July 2011 Accepted: 19 September 2011 Published Online: 24 October 2011
Biointerphases 6, 180 (2011); https://doi.org/10.1116/1.3647506
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  • 1The Interdisciplinary Nanoscience Centre (iNANO), Aarhus University, Ny Munkegade 120, Aarhus, 8000, Denmark
  • 2Industrial Research Institute Swinburne (IRIS), Faculty of Engineering and Industrial Sciences, Advanced Technologies Centre (ATC), Swinburne University of Technology, Burwood Road, Hawthorn, Victoria 3122, Australia
  • a)Author to whom correspondence should be addressed; electronic mail:

    b)Author to whom correspondence should be addressed; electronic mail:

Backfilling a self-assembled monolayer (SAM) of long poly (ethylene glycol) (PEG) with short PEG is a well-known strategy to improve its potential to resist fouling. Here it is shown, using xray photoelectron spectroscopy, contact angle, and atomic force microscopy, that backfilling PEG thiol with oligo (ethylene glycol) (OEG) terminated alkane thiol molecules results in underbrush formation. The authors also confirm the absence of phase separated arrangement, which is commonly observed with backfilling experiments involving SAMs of short chain alkane thiol with long chain alkane thiol. Furthermore, it was found that OEG addition caused less PEG desorption when compared to alkane thiol. The ability of surface to resist fouling was tested through serum adsorption and bacterial adhesion studies. The authors demonstrate that the mixed monolayer with PEG and OEG is better than PEG at resisting protein adsorption and bacterial adhesion, and conclude that backfilling PEG with OEG resulting in the underbrush formation enhances the ability of PEG to resist fouling.
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